Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 396 005

NGF receptor p75 antibody - 396 005

NGFR p75 is a low affinity receptor for both cell survival and cell death
Guinea pig polyclonal purified antibody
Cat. No.: 396 005
Amount: 50 µg
Price: $460.00
Cat. No. 396 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 1:100 up to 1 : 1:500      gallery  
IHC-P: 1 : 1:400 up to 1 : 1:1000      gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 266 to 417 from mouse NGF receptor (UniProt Id: Q9Z0W1)
Reactivity Reacts with: mouse (Q9Z0W1), rat (P07174).
Other species not tested yet.
Data sheet 396_005.pdf
Cat. No.: 396 005
Amount: 50 µg
Price: $460.00
ICC
IHC
IHC
IHC-P
IHC-P
Background
NGF (Nerve Growth Factor) receptor p75, also termed p75NTR, NGFR and CD271, is a low affinity neurotrophin receptor and a single-pass type I transmembrane protein. Its ligands include NGF, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4.
NGF receptor p75 is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, and various neural crest cells and their tumors.
By mediating neurotrophin signals, the receptor appears to play a role in multiple processes, including neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system.
NGF receptor p75 binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed will determine the ligand being recognized and the biological response to its binding.