LAMP3 antibody - 391 004 K.O.

LAMP3 is a glycoprotein of the lysosomal membrane

Guinea pig polyclonal antiserum

Cat. No.: 391 004

Amount: 100 µl

Price: $455.00

Cat. No. 391 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet Dot blot: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 2000 gallery IHC-P: 1 : 1000 up to 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunodetection of target molecules spotted onto a membrane. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to C-terminal residues of mouse LAMP3. (UniProt Id: Q7TST5)
Reactivity	Reacts with: mouse (Q7TST5), rat (Q5XI99). Other species not tested yet.
Specificity	Recognizes LAMP 3, no crossreactivity to LAMP 1, LAMP 2 and LAMP 5. K.O. validated
Remarks	Dot blot: This application was tested with synthetic peptides only.
Data sheet	391_004.pdf

Cat. No.: 391 004

Amount: 100 µl

Price: $455.00

Dot blot

IHC

IHC-P

Background

LAMP 3 (lysosome-associated membrane glycoprotein 3), also referred to as DC-LAMP (dendritic cell LAMP) or CD 208, is a member of the LAMP protein family and a single-pass type I transmembrane protein that can be variably glycosylated. It is specifically expressed in lung type II pneumocytes and by activated human dendritic cells (1, 2).
Recent studies demonstrated high expression of LAMP 3 in a variety of malignancies including squamous cell carcinoma, gastrointestinal cancer, breast cancer, ovarian cancer, and cervical cancer. Its expression has been associated with metastasis and poor overall survival (3, 4, 5).

1 ) de Saint-Vis B et al., Immunity (1998)
2 ) Salaun B et al., Am J Pathol (2004)
3 ) Ozaki K et al., Cancer Res (1998)
4 ) Liao X et al., Int J Mol Sci (2015)
5 ) Wang D et al., Tumour Biol. (2017)

Protocols

IHC

IHC-P

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