RFP (dsRed) antibody - 390 011

RFP is a red fluorescent protein tag

Mouse monoclonal purified IgG

Cat. No.: 390 011

Amount: 100 µg

Price: $420.00

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Cat. No. 390 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	115D6
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 225 from sea anemone DsRed (UniProt Id: Q9U6Y8)
Specificity	Recognizes mRFP, mCherry, mOrange2, and mScarlet. Does not detect dsRed and tdTomato.
Data sheet	Data Sheet 390_011 Safety Data Sheet (SDS)

References for RFP - 390 011

Stage-Dependent Effects of "Too Little and Too Much" Medial Prefrontal Activity on Reversal Learning in Rats: Functional Inhibition Impairs Early, Whereas Neural Disinhibition Impairs Late Reversals.
Renström JG, Taylor CJL, Grasmeder Allen R, O'Hara L, Loayza J, Juty J, Moran PM, von Heimendahl M, Deiana S, Du Hoffman J, Stevenson CW, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2026) 4636: . 390 011 IHC; tested species: rat

Cat. No.: 390 011

Amount: 100 µg

Price: $420.00

ICC
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Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D et al., Genesis (2010)
2 ) Shaner NC et al., Nat. Biotechnol. (2004)
3 ) Labas YA et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
4 ) Fradkov AF et al., FEBS Lett. (2000)

Protocols

ICC

Fixed Cells

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