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Ankyrin G antibody - 386 011

AnkyrinG is a membrane-cytoskeleton linker
Mouse monoclonal purified IgG
Cat. No.: 386 011
Amount: 100 µg
Price: $415.00
Cat. No. 386 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
Clone SY-305D4
Subtype IgG1 (λ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity Reacts with: mouse (G5E8K5-1), rat (O70511-1).
Other species not tested yet.
Specificity Ankyrin G, detects all described splice variants.
Remarks

IHC-Fr: Fixation with acetone, PFA/formaldehyde is recommended. Acetone-methanol or methanol fixation result in weaker signal intensity.

Signal intensities as follows: acetone > PFA >> acetone-methanol > methanol.

Data sheet 386_011.pdf
Cat. No.: 386 011
Amount: 100 µg
Price: $415.00
Background
Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).