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Home > Products > 379 003

BGT1 antibody - 379 003

BGT1 is a sodium- and chloride-dependent betaine-GABA transporter
Rabbit polyclonal purified antibody
Cat. No.: 379 003
Amount: 50 µg
Price: $375.00
Cat. No. 379 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 567 to 614 from human BGT (UniProt Id: P48065)
Reactivity Reacts with: human (P48065).
Other species not tested yet.
Specificity Specific for BGT 1
Remarks

WB:  BGT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 379_003.pdf
Cat. No.: 379 003
Amount: 50 µg
Price: $375.00
WB
Background
The sodium- and chloride-dependent betaine-GABA transporter 1 (BGT 1) is a member of the neurotransmitter-sodium-symporter transporter family. It mediates cellular uptake of betaine and GABA in a sodium- and chloride-dependent process.
BGT 1 is involved in betaine transport in the kidney medulla to withstand hyperosmolarity. BGT 1 also transports GABA but its exact role in brain is not yet fully understood.