TRIM46 antibody - 377 011 K.O.

TRIM46 is involved in establishing neuronal polarity

Mouse monoclonal purified IgG

Cat. No.: 377 011

Amount: 100 µg

Price: $420.00

Cat. No. 377 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 250 gallery IHC: 1 : 100 gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	264D12
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 561 from rat Trim46 (UniProt Id: A0A0G2JXN2)
Epitop	AA 1 to 114 from rat Trim46 (UniProt Id: A0A0G2JXN2)
Reactivity	Reacts with: mouse (Q7TNM2), rat (A0A0G2JXN2). Other species not tested yet. Predicted to cross-react with human (Q7Z4K8) due to high sequence homology.
Specificity	K.O. validated
Data sheet	Datasheet 377_011

Cat. No.: 377 011

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

The tripartite motif containing protein TRIM46, also referred to as TRIFIC, is a member of the C-I TRIM family, a subfamily of the RBCC (N-terminal RING finger/B-box/coiled coil)/TRIM superfamily.
TRIM46 localizes to newly specified axons and, at later stages, to the axon initial segment (AIS). By forming closely spaced parallel microtubule bundles it plays a crucial role in the initial polarization of neuronal cells.

1 ) van Beuningen SF et al., Neuron (2015)

Protocols

ICC

Fixed Cells

IHC

IHC-P

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