Cat. No.: 377 009
Amount: 50 µg
Price:
$415.00
Cat. No. 377 009 |
50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 400 up to 1 : 2000 gallery |
Clone | Ch264D12 |
Subtype | IgY (λ light chain) |
Immunogen | Recombinant protein comprising a major part of the protein (UniProt Id: A0A0G2JXN2) |
Epitop |
Epitop: AA 1 to 114 from rat Trim46 (UniProt Id: A0A0G2JXN2) |
Reactivity |
Reacts with: mouse (Q7TNM2), rat (A0A0G2JXN2). Other species not tested yet. |
Specificity | K.O. validated |
Remarks |
This antibody is a chimeric antibody based on the monoclonal mouse antibody 264D12. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells. |
Data sheet | 377_009.pdf |
The tripartite motif containing protein TRIM46, also referred to as TRIFIC, is a member of the C-I TRIM family, a subfamily of the RBCC (N-terminal RING finger/B-box/coiled coil)/TRIM superfamily.
TRIM46 localizes to newly specified axons and, at later stages, to the axon initial segment (AIS). By forming closely spaced parallel microtubule bundles it plays a crucial role in the initial polarization of neuronal cells.