Tailor-made Antibodies
and Tools for Life Science

TRIM46 antibody - 377 009 K.O.

TRIM46 is involved in establishing neuronal polarity
Chicken monoclonal recombinant IgY
Cat. No.: 377 009
Amount: 50 µg
Price: $420.00
Cat. No. 377 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 400 up to 1 : 2000 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
IHC-G: 1 : 500 (see remarks) gallery  
Clone Ch264D12
Subtype IgY (λ light chain)
Immunogen Recombinant protein comprising a major part of the protein (UniProt Id: A0A0G2JXN2)
Epitop AA 1 to 114 from rat Trim46 (UniProt Id: A0A0G2JXN2)
Reactivity Reacts with: mouse (Q7TNM2), rat (A0A0G2JXN2).
Other species not tested yet.
Predicted to cross-react with human (Q7Z4K8) due to high sequence homology.
Specificity K.O. validated
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody 264D12. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
IHC-Fr: Fixation with acetone, PFA/formaldehyde is recommended. Fixation with acetone-methanol or methanol result in a weaker signal intensity.

Signal intensities as follows: acetone > PFA >> acetone-methanol > methanol.
IHC-G: 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.

Data sheet 377_009.pdf
Cat. No.: 377 009
Amount: 50 µg
Price: $420.00