Synaptic Systems - Numblike

Cat. No.: 374 003

Amount: 50 µg

Price: $375.00

WB

ICC

IHC-Fr

Background

Numb proteins (Numblike and Numb) display a complex pattern of functions such as the control of asymmetric cell division, cell fate choice, endocytosis, cell adhesion, and cell migration. They have been shown to inhibit Notch signaling by stimulating endocytosis of Notch.
Numb and Numblike have at least partially distinct functions. Numblike is a negative regulator of the NF-κB signaling pathway by abrogating TRAF5-induced activation of NF-κB. Recently, Numblike was implicated as a physiologically relevant target of microRNA miR-34a in neural progenitor cells allowing for enhanced Notch signaling and inhibition of neuronal differentiation.
Numb and Numblike are essential in maintaining neural progenitor cells during early neurogenesis by allowing cells to choose progenitor over neuronal fates. They were recently also discovered to be involved in cardiac morphogenesis.

1 ) Wu M et al., Biomol Concepts (2015)
2 ) Shenje LT et al., Elife (2014)
3 ) Yingjie L et al., Tumour Biol. (2013)
4 ) Yang J et al., Cardiovasc. Res. (2012)
5 ) Fineberg SK et al., PLoS ONE (2012)
6 ) Tao T et al., Mol. Biol. Cell (2012)
7 ) Liu L et al., Biochem. Biophys. Res. Commun. (2011)
8 ) Gulino A et al., Exp. Cell Res. (2010)
9 ) Rasin MR et al., Nat. Neurosci. (2007)
10 ) Kuo CT et al., Cell (2006)

Protocols

WB

ICC

IHC-Fr

OTHER

Preabsorbtion

Other products for this target

Cat. No. 374 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 gallery IHC: not tested yet IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 579 to 595 from mouse Numbl (UniProt Id: O08919)
Reactivity	Reacts with: rat (A1L1I3), mouse (O08919). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	374-0P
Remarks	IHC-Fr: PFA fixation is recommended.
Data sheet	374_003.pdf

Numblike antibody - 374 003 K.O.