|Cat. No. 374 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 100 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 579 to 595 from mouse Numbl (UniProt Id: O08919)|
Reacts with: rat (A1L1I3), mouse (O08919).
Other species not tested yet.
|Specificity||Specific for Numblike. K.O.|
|Matching control protein/peptide||374-0P|
Numb proteins (Numblike and Numb) display a complex pattern of functions such as the control of asymmetric cell division, cell fate choice, endocytosis, cell adhesion, and cell migration. They have been shown to inhibit Notch signaling by stimulating endocytosis of Notch.
Numb and Numblike have at least partially distinct functions. Numblike is a negative regulator of the NF-κB signaling pathway by abrogating TRAF5-induced activation of NF-κB. Recently, Numblike was implicated as a physiologically relevant target of microRNA miR-34a in neural progenitor cells allowing for enhanced Notch signaling and inhibition of neuronal differentiation.
Numb and Numblike are essential in maintaining neural progenitor cells during early neurogenesis by allowing cells to choose progenitor over neuronal fates. They were recently also discovered to be involved in cardiac morphogenesis.