Synaptic Systems

Cat. No.: 373 003

Amount: 50 µg

Price: $375.00

WB

ICC

IHC-Fr

Background

Numb proteins (Numb and Numblike) display a complex pattern of functions such as the control of asymmetric cell division, cell fate choice, endocytosis, cell adhesion, and cell migration. Numb has been shown to inhibit Notch signaling by recruiting α-Adaptin and stimulating endocytosis of Notch. It was also demonstrated that Numb helps activate the tumor suppressor p53, suggesting that loss of Numb in cancerous cells would result in both the activation of the potential oncogene Notch and the diminution of tumor suppression by p53. Numb is itself regulated via ubiquitinylation.
Numb and Numblike are redundant but essential in maintaining neural progenitor cells during early neurogenesis by allowing cells to choose progenitor over neuronal fates. Numb and Numblike were also recently discovered to be involved in cardiac morphogenesis.
Four isoforms of mammalian Numb are described with predicted molecular masses of 65, 66, 71, and 72 kDa.

1 ) Wu M et al., Biomol Concepts (2015)
2 ) Shenje LT et al., Elife (2014)
3 ) Flores AN et al., Nat Rev Urol (2014)
4 ) Gulino A et al., Exp. Cell Res. (2010)
5 ) Carter S et al., Genome Biol. (2008)
6 ) Zhou Y et al., Cell (2007)
7 ) Kuo CT et al., Cell (2006)
8 ) Petersen PH et al., Nat. Neurosci. (2004)
9 ) Johnson JE et al., Trends Neurosci. (2003)
10 ) Jafar-Nejad H et al., Dev. Cell (2002)

Protocols

WB

ICC

IHC-Fr

OTHER

Preabsorbtion

Other products for this target

Cat. No. 373 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 625 to 644 from mouse Numb (UniProt Id: Q9QZS3)
Reactivity	Reacts with: rat (Q2LC84), mouse (Q9QZS3). Other species not tested yet.
Specificity	Specific for Numb. Recognizes all four isoforms. K.O. validated
Matching control protein/peptide	373-0P
Remarks	ICC: Short fixation of only 5 min with 4% PFA ias recommended. IHC-Fr: Fixation for 10 min with 4% PFA at RT is recommended.
Data sheet	373_003.pdf

Numb antibody - 373 003 K.O.