CD34 antibody - 370 011

CD34 is a hematopoietic progenitor cell antigen

Mouse monoclonal purified IgG

Cat. No.: 370 011

Amount: 100 µg

Price: $420.00

Cat. No. 370 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not recommended FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone	171G9
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 32 to 291 from human CD34 (UniProt Id: P28906)
Reactivity	Reacts with: human (P28906). Other species not tested yet.
Data sheet	Datasheet 370_011

Cat. No.: 370 011

Amount: 100 µg

Price: $420.00

Background

CD 34 is a single pass trans-membrane glycoprotein that localizes to the surface of hematopoetic progenitor cells. The presence of CD 34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes.

1 ) Sagha M et al., Methods Mol. Biol. (2016)
2 ) Middleton J et al., J. Pathol. (2005)
3 ) Naruse K et al., J. Pathol. (1999)
4 ) Molgaard HV et al., Leukemia (1989)
5 ) Sutherland DR et al., Leukemia (1988)