DOPA decarboxylase antibody - 369 011

DOPA decarboxylase is an enzyme for dopamine, serotonin, and trace amine biosynthesis

Mouse monoclonal purified IgG

Cat. No.: 369 011

Amount: 100 µg

Price: $415.00

Cat. No. 369 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 5000 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	126F10
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 480 from mouse DDC (UniProt Id: O88533)
Reactivity	Reacts with: mouse (O88533), rat (P14173). Other species not tested yet.
Data sheet	369_011.pdf

Cat. No.: 369 011

Amount: 100 µg

Price: $415.00

IHC

IHC-P

Background

DOPA decarboxylase, also referred to as aromatic L-amino acid decarboxylase, AADC or DDC, is the second enzyme in the biosynthesis of dopamine and serotonin. In addition, it is responsible for the synthesis of the trace amines phenylethylamine, p-tyramine, and tryptamine, which are considered to act as neuromodulators.
DDC is expressed in the central nervous system, but has also been detected in several tissues such as liver, kidney, small intestine, adrenal gland and blood vessels.
The inhibition of DDC is used for the treatment of Parkinson's disease.

1 ) Haycock JW et al., J. Neurochem. (2003)
2 ) Burkhard P et al., Nat. Struct. Biol. (2001)
3 ) Berry MD et al., Neurochem. Res. (1996)
4 ) Ishii S et al., J. Biochem. (1996)
5 ) Albert VR et al., Proc. Natl. Acad. Sci. U.S.A. (1992)
6 ) Jaeger CB et al., Neuroscience (1984)

Protocols

IHC

IHC-P

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