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Home > Products > 367 008

Glutamine synthetase antibody - 367 008

GS is an enzyme for glutamine synthesis and ammonia detoxification, a marker for astrocytes
Rabbit monoclonal recombinant IgG
Cat. No.: 367 008
Amount: 50 µg
Price: $415.00
Cat. No. 367 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 2000 gallery  
IHC-P: 1 : 10000 up to 1 : 20000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone Rb250C4
Subtype IgG1 (κ light chain)
Immunogen Full-length recombinant mouse Glutamine synthetase (UniProt Id: P15105)
Reactivity Reacts with: rat (P09606), mouse (P15105).
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 250C4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet 367_008.pdf
Cat. No.: 367 008
Amount: 50 µg
Price: $415.00
WB
ICC
ICC
IHC
IHC
IHC
IHC-P
IHC-P
Background
Glutamine synthetase, also referred to as Glutamate-ammonia ligase or GS, is an enzyme that catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. It is present predominantly in brain, kidneys, and liver. In the brain, it is particularly found in astrocytes.
Glutamine synthetase plays a pivotal role in glutamate and glutamine homoeostasis, and it is largely responsible for the removal of both blood-derived and metabolically generated ammonia, preventing neurotoxicity. It is also a key enzyme in the recycling of the neurotransmitter glutamate.
Several studies indicated that the expression, distribution, and activity of brain glutamine synthetase is altered in some brain disorders, including Alzheimer's disease, schizophrenia, depression, suicidality, and mesial temporal lobe epilepsy (MTLE).
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