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Home > Products > 347 404

SOX10 antibody - 347 404

SOX10 is a transcription factor involved in the regulation of progenitor cell specification and terminal differentiation
Guinea pig polyclonal antiserum
Cat. No.: 347 404
Amount: 100 µl
Price: $365.00
Cat. No. 347 404 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 2000 up to 1 : 4000 (AP-staining) gallery  
IP: not tested yet
ICC: yes gallery  
IHC: 1 : 2000 gallery  
IHC-P: yes gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the amino terminus of mouse SOX10 (UniProt Id: Q04888)
Reactivity Reacts with: rat (O55170), mouse (Q04888).
Other species not tested yet.
Data sheet 347_404.pdf
Cat. No.: 347 404
Amount: 100 µl
Price: $365.00
WB
ICC
IHC
IHC
IHC
IHC
IHC-P
IHC-P
IHC-P
Background

SRY-Box Transcription Factor 10 (SOX10) belongs to a family of transcription factors characterized by a sequence-specific DNA-binding domain known as the high mobility group-box (HMGB). Members of the SOX family belong to the HMGB subfamily and represent a class of genes expressed during early embryonic development that are involved in cell fate specifications of progenitor cells and terminal cell differentiation in a variety of cell type linages (1-3). SOX10 is a key regulator in early development of neuronal crest cells that give rise to peripheral glial cells (4), melanocytes, cells of the enteric nervous system or chromaffin cells. In the central nervous system SOX10 is present in oligodendrocyte progenitors (OPCs) and remains expressed throughout oligodendrocyte (OL) development and linage. During OL development SOX10 exerts critical functions in terminal OL differentiation and myelin gene expression (5). SOX10 mutations have been reported to Waardenburg syndrome type 4, peripheral demyelinating neuropathy, central demyelinating leukodystrophy, Hirschsprung disease (PCWH), cancer and others.