c-Myc antibody - 343 008

c-Myc is a protein involved in the control of cell proliferation

Rabbit monoclonal recombinant IgG

Cat. No.: 343 008

Amount: 100 µg

Price: $420.00

Cat. No. 343 008	100 µg purified recombinant IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: yes IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb9E10
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 408 to 432 from human c-Myc (UniProt Id: P01106)
Reactivity	Reacts with: human (P01106). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 9E10. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells.
Data sheet	343_008.pdf

References for c-Myc - 343 008

Novel Functional Properties of Missense Mutations in the Glycine Receptor β Subunit in Startle Disease.
Piro I, Eckes AL, Kasaragod VB, Sommer C, Harvey RJ, Schaefer N, Villmann C
Frontiers in molecular neuroscience (2021) 14: 745275. 343 008 ICC; tested species: mouse

The Rab5 activator RME-6 is required for amyloid precursor protein endocytosis depending on the YTSI motif.
Eggert S, Gruebl T, Rajender R, Rupp C, Sander B, Heesch A, Zimmermann M, Hoepfner S, Zentgraf H, Kins S
Cellular and molecular life sciences : CMLS (2020) : . 343 008 ICC; tested species: human

Cat. No.: 343 008

Amount: 100 µg

Price: $420.00

ICC

Background

The c-Myc oncogene (p62 c-Myc) is a nuclear protein that is involved in the control of normal cellular proliferation and differentiation. Deregulated expression of c-Myc induces apoptosis in different cell types.
The C-terminal c-Myc amino acids are widely used in combination with eukaryotic expression vectors encoding proteins with c-Myc epitope tag.

1 ) Kübler K et al., Oncotarget (2015)
2 ) Evan GI et al., Mol. Cell. Biol. (1985)
3 ) Dalla-Favera R et al., Proc. Natl. Acad. Sci. U.S.A. (1982)
4 ) Vennstrom B et al., J. Virol. (1982)

Protocols

Standard protocol

ICC

IHC

IHC-P

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