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SATB2 antibody - 327 003

SATB2 is a transcription factor involved in neuronal differentiation
Rabbit polyclonal purified antibody
Cat. No.: 327 003
Amount: 50 µg
Price: $375.00
Cat. No. 327 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 718 to 733 from mouse SATB2 (UniProt Id: Q8VI24)
Reactivity Reacts with: rat (D3ZJ19), mouse (Q8VI24).
Other species not tested yet.
Data sheet 327_003.pdf
Cat. No.: 327 003
Amount: 50 µg
Price: $375.00
Background

The Special AT-rich sequence-binding protein 2 or SATB 2 is a transcription factor required for the initiation of the genetic program for the upper-layer neurons (UL1).
Together with Ctip 2, Coup-TFI, and Fezf 2 it is involved in the fine tuned sequential formation and specification of the different excitatory neuron populations forming the definitive six-layered cortical structure.