Synaptic Systems - Doublecortin

Cat. No.: 326 004

Amount: 100 µl

Price: $365.00

Cat. No. 326 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not recommended IHC: not recommended IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 271 to 366 from mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity	Reacts with: rat (Q9ESI7), mouse (Q6PGI2). Other species not tested yet.
Data sheet	326_004.pdf

References for Doublecortin - 326 004

Azithromycin preserves adult hippocampal neurogenesis and behavior in a mouse model of sepsis.
Rodríguez-Moreno CB, Cañeque-Rufo H, Flor-García M, Terreros-Roncal J, Moreno-Jiménez EP, Pallas-Bazarra N, Bressa C, Larrosa M, Cafini F, Llorens-Martín M
Brain, behavior, and immunity (2024) 117: 135-148. 326 004 IHC; tested species: mouse

Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 004 IHC; tested species: mouse

Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 326 004 IHC; tested species: rat

Impaired p53-Mediated DNA Damage Response Contributes to Microcephaly in Nijmegen Breakage Syndrome Patient-Derived Cerebral Organoids.
Martins S, Erichsen L, Datsi A, Wruck W, Goering W, Chatzantonaki E, de Amorim VCM, Rossi A, Chrzanowska KH, Adjaye J
Cells (2022) 115: . 326 004 IHC; tested species: human

Cat. No.: 326 004

Amount: 100 µl

Price: $365.00

IHC-P

Background

Doublecortin or DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Mutations in the gene encoding this protein have been linked to the double cortex syndrome.
Doublecortin is highly expressed in neuronal cells of fetal brain but absent from other tissues. In the adult dentate gyrus it is only expressed in cells contributing to adult neurogenesis.

1 ) Tang SK et al., Neuroscience (2014)
2 ) Plümpe T et al., BMC Neurosci (2006)
3 ) Moores CA et al., EMBO J. (2006)
4 ) Francis F et al., Neuron (1999)
5 ) Gleeson JG et al., Cell (1998)

Protocols

Standard protocol

IHC-P

Other products for this target

Doublecortin antibody - 326 004

References for Doublecortin - 326 004