Tau antibody - 314 111

Tau is a neuronal microtubule associated protein

Mouse monoclonal purified IgG

Cat. No.: 314 111

Amount: 100 µg

Price: $415.00

Cat. No. 314 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 1000 up to 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	110G12
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 44 from human TauE (UniProt Id: P10636-7)
Reactivity	Reacts with: human (P10636-7). Other species not tested yet.
Specificity	This antibody binds phosphorylated and non-phosphorylated tau proteins. The sequence used for immunization is present in all splice variants except human TauA (UniProt Id: P10636-3)
Remarks	This antibody is highly recommended for human tissue.
Data sheet	314_111.pdf

Cat. No.: 314 111

Amount: 100 µg

Price: $415.00

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.

1 ) Thies E et al., J. Neurosci. (2007)
2 ) Avila J et al., J. Biomed. Biotechnol. (2006)
3 ) Duka T et al., FASEB J. (2006)
4 ) Black MM et al., J. Neurosci. (1996)
5 ) Mandell JW et al., J. Neurosci. (1996)
6 ) Hirokawa N et al., J. Cell Biol. (1988)
7 ) Connolly JA et al., Proc. Natl. Acad. Sci. U.S.A. (1977)
8 ) Witman GB et al., Proc. Natl. Acad. Sci. U.S.A. (1976)

Protocols

IHC

IHC-P

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