|Cat. No. 314 011||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 200 up to 1 : 500 gallery
IHC: 1 : 100 gallery
IHC-P/FFPE: 1 : 500 gallery
|Subtype||IgG2a (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 3 to 214 from mouse Tau-D (UniProt Id: P10637-5)|
Epitop: AA 3 to 214 from mouse Tau-D (UniProt Id: P10637-5)
Reacts with: rat (P19332), mouse (P10637).
Weaker signal: human (P10636).
No signal: zebrafish.
Other species not tested yet.
|Specificity||Specific for tau. Sequence used for immunization is present in all splice variants.|
|Matching control protein/peptide||314-0P|
WB: Detects the mouse protein with much greater sensitivity than the rat protein.
There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.