Tau antibody - 314 008

Tau is a neuronal microtubule associated protein

Rabbit monoclonal recombinant IgG

Cat. No.: 314 008

Amount: 50 µg

Price: $415.00

Cat. No. 314 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 4000 up to 1 : 20000 gallery DNA-PAINT: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Clone	Rb248E5
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)
Reactivity	Reacts with: rat (P19332), mouse (P10637). Weaker signal: human (P10636). No signal: zebrafish. Other species not tested yet.
Specificity	This antibody binds phosphorylated and non-phosphorylated tau proteins. The sequence used for immunization is present in all splice variants except human TauA (UniProt Id: P10636-3).
Matching control protein/peptide	314-0P
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 248E5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet	314_008.pdf

References for Tau - 314 008

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 314 008 DNA-PAINT; tested species: rat

Cat. No.: 314 008

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.

1 ) Thies E et al., J. Neurosci. (2007)
2 ) Avila J et al., J. Biomed. Biotechnol. (2006)
3 ) Duka T et al., FASEB J. (2006)
4 ) Black MM et al., J. Neurosci. (1996)
5 ) Mandell JW et al., J. Neurosci. (1996)
6 ) Hirokawa N et al., J. Cell Biol. (1988)
7 ) Connolly JA et al., Proc. Natl. Acad. Sci. U.S.A. (1977)
8 ) Witman GB et al., Proc. Natl. Acad. Sci. U.S.A. (1976)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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