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Tau - 314 008

A microtubule associated protein
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 314 008
Amount: 50 µg
Price: 415.00 €
Cat. No. 314 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 4000 up to 1 : 20000 gallery  
Clone Rb248E5
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 214 from mouse Tau-D (UniProt Id: P10637-5)
Reactivity Reacts with: rat (P19332), mouse (P10637).
Weaker signal: human (P10636).
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for tau. Sequence used for immunization is present in all splice variants.
Matching control protein/peptide 314-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 248E5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 314_008.pdf
Cat. No.: 314 008
Quantity: 50 µg
Price: 415.00 €
Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.