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Nestin antibody - 312 111

Nestin is a class VI intermediate filament
Mouse monoclonal purified IgG
Cat. No.: 312 111
Amount: 100 µg
Price: $415.00
Cat. No. 312 111 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in TRIS. Then aliquot and store at -20°C until use.
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500
IHC-P: 1 : 500 up to 1 : 2000 gallery  
ELISA: yes
Clone JP39
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 1618 from human Nestin (UniProt Id: P48681)
Reactivity Reacts with: human (P48681).
Other species not tested yet.
Data sheet 312_111.pdf
Cat. No.: 312 111
Amount: 100 µg
Price: $415.00

Nestin is a type IV intermediate filament and is expressed in early embryonic neuroepithelial stem cells during CNS development (1). The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation (2). Antibodies against Nestin can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
During Neuroinfflamation and ischemia, Nestin is upregulated in different CNS cell types (2).
Expression has also been reported for proliferative endothelial cells of various tissues like newly formed blood vessels in brain (3), glomeruli of kidney (4) and skin (5).