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Home > Products > 312 111

Nestin antibody - 312 111

Nestin is a class VI intermediate filament
Mouse monoclonal purified IgG
Cat. No.: 312 111
Amount: 100 µg
Price: $415.00
Cat. No. 312 111 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in TRIS. Then aliquot and store at -20°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500
IHC-P: 1 : 500 up to 1 : 2000 gallery  
ELISA: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone JP39
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 1618 from human Nestin (UniProt Id: P48681)
Reactivity Reacts with: human (P48681).
Other species not tested yet.
Data sheet 312_111.pdf
Cat. No.: 312 111
Amount: 100 µg
Price: $415.00
WB
ICC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
Background

Nestin is a type VI intermediate filament and is expressed in early embryonic neuroepithelial stem cells during CNS development (1). The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation (2). Antibodies against Nestin can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
During neuroinflammation and ischemia, Nestin is upregulated in different CNS cell types (2).
Expression has also been reported for proliferative endothelial cells of various tissues like newly formed blood vessels in brain (3), glomeruli of kidney (4) and skin (5). Moreover, Nestin has garnered significant attention in oncology, as its re-expression is associated with various cancers, including glioblastomas and melanomas (6).
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