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Nestin - 312 111

A class VI intermediate filament
Monoclonal mouse purified IgG
Cat. No.: 312 111
Amount: 100 µg
Price: 415.00 €
Cat. No. 312 111 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in TRIS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
ELISA: yes
Clone JP39
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 1618 from human Nestin (UniProt Id: P48681)
Epitop Epitop: AA 1 to 1618 from human Nestin (UniProt Id: P48681)
Reactivity Reacts with: human (P48681).
Other species not tested yet.
Specificity Specific for human nestin.
Remarks

Differences between clone JP 63 and clone JP39 in application or specificity not determined yet.

Data sheet 312_111.pdf
Cat. No.: 312 111
Quantity: 100 µg
Price: 415.00 €
Background
Nestin is expressed in early embryonic neuroepithelial stem cells during development of the CNS. The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation. Antibodies against this protein can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
Nestin is also a useful marker-protein for brain tumors of both glial and neuronal lineage.