Synaptic Systems

Cat. No.: 312 005

Amount: 50 µg

Price: $370.00

ICC

Background

Nestin is a type VI intermediate filament and is expressed in early embryonic neuroepithelial stem cells during CNS development (1). The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation (2). Antibodies against Nestin can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
During neuroinflammation and ischemia, Nestin is upregulated in different CNS cell types (2).
Expression has also been reported for proliferative endothelial cells of various tissues like newly formed blood vessels in brain (3), glomeruli of kidney (4) and skin (5). Moreover, Nestin has garnered significant attention in oncology, as its re-expression is associated with various cancers, including glioblastomas and melanomas (6).

1 ) McKay R et al., Science (1997)
2 ) Krishnasamy S et al., J Neuroinflammation (2017)
3 ) Suzuki S et al., J Histochem Cytochem (2010)
4 ) Su W et al., Am. J. Physiol. Regul. Integr. Comp. Physiol. (2007)
5 ) Wang Y et al., Cell Biol Int (2006)
6 ) Wang Q et al., Cancer Cell (2017)

Protocols

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Other products for this target

Cat. No. 312 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: yes (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Nestin (UniProt Id: Q6P5H2)
Reactivity	Reacts with: rat (P21263), mouse (Q6P5H2). Other species not tested yet.
Remarks	ICC: PFA, glyoxal and methanol fixation are possible
Data sheet	312_005.pdf

Nestin antibody - 312 005