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Nestin antibody - 312 005

Nestin is a class VI intermediate filament
Guinea pig polyclonal purified antibody
Cat. No.: 312 005
Amount: 50 µg
Price: $370.00
Cat. No. 312 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: yes (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse Nestin (UniProt Id: Q6P5H2)
Reactivity Reacts with: rat (P21263), mouse (Q6P5H2).
Other species not tested yet.
Remarks

ICC: PFA, glyoxal and methanol fixation are possible

Data sheet 312_005.pdf
Cat. No.: 312 005
Amount: 50 µg
Price: $370.00
Background

Nestin is a type VI intermediate filament and is expressed in early embryonic neuroepithelial stem cells during CNS development (1). The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation (2). Antibodies against Nestin can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
During neuroinflammation and ischemia, Nestin is upregulated in different CNS cell types (2).
Expression has also been reported for proliferative endothelial cells of various tissues like newly formed blood vessels in brain (3), glomeruli of kidney (4) and skin (5).