Cat. No.: 312 005
Amount: 50 µg
Price:
$370.00
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| Cat. No. 312 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: not tested yet ICC: yes (see remarks) gallery IHC: not tested yet IHC-P: not tested yet |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of mouse Nestin (UniProt Id: Q6P5H2) |
| Reactivity |
Reacts with: rat (P21263), mouse (Q6P5H2). Other species not tested yet. |
| Remarks |
ICC: PFA, glyoxal and methanol fixation are possible |
| Data sheet | 312_005.pdf |
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Nestin is a type VI intermediate filament and is expressed in early embryonic neuroepithelial stem cells during CNS development (1). The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation (2). Antibodies against Nestin can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
During neuroinflammation and ischemia, Nestin is upregulated in different CNS cell types (2).
Expression has also been reported for proliferative endothelial cells of various tissues like newly formed blood vessels in brain (3), glomeruli of kidney (4) and skin (5).