|Cat. No. 311 018||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: yes (see remarks)
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 200 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 175 from human MLC-2A (UniProt Id: Q01449)|
Reacts with: human (Q01449), rat, mouse (Q9QVP4).
No signal: chicken.
Other species not tested yet.
|Specificity||Specific for MLC-2A, no cross-reactivity to MLC-2V|
|Matching control protein/peptide||311-0P|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 56F5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
ICC: Methanol fixation is recommended
During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2A is only present in the atrium while MLC-2V is exclusively expressed in the ventricle. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
This monoclonal antibody is a useful tool to distinguish between ventricle and atrium specific cardiomyocytes.