Synaptic Systems

Cat. No.: 307 011

Amount: 100 µg

Price: $420.00

Cat. No. 307 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 2000 (AP staining) gallery IP: yes ICC: 1 : 100 gallery IHC: not tested yet IHC-P: 1 : 200 gallery
Clone	164D4
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 572 from human Zyxin (UniProt Id: Q15942)
Epitop	AA 352 to 357 from human Zyxin (UniProt Id: Q15942)
Reactivity	Reacts with: human (Q15942), rat, mouse (Q62523), hamster. Other species not tested yet.
Data sheet	307_011.pdf

References for Zyxin - 307 011

Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1).
Endres M, Kneitz S, Orth MF, Perera RK, Zernecke A, Butt E
Oncotarget (2016) 739: 64244-64259. 307 011 WB

mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes.
Vollenbröker B, George B, Wolfgart M, Saleem MA, Pavenstädt H, Weide T
American journal of physiology. Renal physiology (2009) 2962: F418-26. 307 011 WB, ICC

Targeted disruption of the mouse Lipoma Preferred Partner gene.
Vervenne HB, Crombez KR, Delvaux EL, Janssens V, Van de Ven WJ, Petit MM
Biochemical and biophysical research communications (2009) 3792: 368-73. 307 011 WB

Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis.
Mori M, Nakagami H, Koibuchi N, Miura K, Takami Y, Koriyama H, Hayashi H, Sabe H, Mochizuki N, Morishita R, Kaneda Y, et al.
Molecular biology of the cell (2009) 2013: 3115-24. 307 011 WB, ICC

TES is a novel focal adhesion protein with a role in cell spreading.
Coutts AS, MacKenzie E, Griffith E, Black DM
Journal of cell science (2003) 116Pt 5: 897-906. 307 011 WB, ICC

The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions.
Garvalov BK, Higgins TE, Sutherland JD, Zettl M, Scaplehorn N, Köcher T, Piddini E, Griffiths G, Way M
The Journal of cell biology (2003) 1611: 33-9. 307 011 WB, ICC

Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Rottner K, Krause M, Gimona M, Small JV, Wehland J
Molecular biology of the cell (2001) 1210: 3103-13. 307 011 WB, ICC

Cat. No.: 307 011

Amount: 100 µg

Price: $420.00

ICC

IHC-P

Background

Zyxin is a LIM domain-containing cytoskeletal protein crucial for mechanotransduction, actin cytoskeleton remodeling, and cell adhesion. Predominantly localized at focal adhesions and actin stress fibers, zyxin serves as a scaffold for proteins involved in signal transduction and mechanical force adaptation. Its N-terminal proline-rich domain enables interactions with SH3 domain-containing proteins, while its C-terminal LIM domains facilitate protein-protein interactions, contributing to cytoskeletal integrity and gene expression regulation (1).
Beyond its general cytoskeletal functions, zyxin plays a pivotal role in vascular biology by maintaining endothelial barrier function and regulating actin dynamics in response to shear stress, thus impacting vascular homeostasis and angiogenesis, as well as a role in inflammatory diseases such as psoriasis and inflammatory bowel disease (2). In brain ischemia, zyxin modulates neurovascular responses, influencing cell survival and tissue recovery following stroke-induced damage. It has been shown to regulate blood-brain barrier integrity and contribute to neuronal cytoskeletal reorganization under ischemic stress (3).
Moreover, zyxin has emerged as a significant player in tumorigenesis, where it exerts context-dependent effects on cancer progression. While it supports tumor cell migration and invasion by modulating actin polymerization and focal adhesion turnover, it also interacts with signaling pathways that can either suppress or promote malignancy, depending on tumor type and the cellular microenvironment (4). Understanding zyxin’s dual role in tumor biology provides potential therapeutic insights into targeting cytoskeletal dynamics in cancer treatment.

1 ) Li B et al., J. Biol. Chem. (2001)
2 ) Sun Z et al., Front Physiol (2012)
3 ) Kang X et al., Front Physiol (2021)
4 ) Wu Z et al., Front Mol Biosci (2024)

Protocols

Zyxin antibody - 307 011

References for Zyxin - 307 011