Synaptic Systems - alpha-Tubulin

Cat. No.: 302 217

Amount: 100 µg

Price: $420.00

Cat. No. 302 217	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 gallery IHC: 1 : 400 gallery IHC-P: not tested yet
Clone	37B5
Subtype	IgG1
Immunogen	Synthetic peptide corresponding to AA 443 to 449 from rat α-Tubulin 1A (UniProt Id: P68370-1)
Reactivity	Reacts with: mammals, chicken. Other species not tested yet.
Specificity	Specific for α-tubulin.
Data sheet	302_217.pdf

References for alpha-Tubulin - 302 217

Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 217 WB, ICC; tested species: rat

Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors.
Luchkina NV, Huupponen J, Clarke VR, Coleman SK, Keinänen K, Taira T, Lauri SE
Proceedings of the National Academy of Sciences of the United States of America (2014) 11111: 4321-6. 302 217 WB; tested species: mouse,rat

Ongoing intrinsic synchronous activity is required for the functional maturation of CA3-CA1 glutamatergic synapses.
Huupponen J, Molchanova SM, Lauri SE, Taira T
Cerebral cortex (New York, N.Y. : 1991) (2013) 2311: 2754-64. 302 217 WB; tested species: rat

Cat. No.: 302 217

Amount: 100 µg

Price: $420.00

ICC

IHC

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

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alpha-Tubulin antibody - 302 217

References for alpha-Tubulin - 302 217