Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

alpha-Tubulin antibody - 302 217

alpha-Tubulin is a major cytoskeleton protein
Rat monoclonal purified IgG
Cat. No.: 302 217
Amount: 100 µg
Price: $415.00
Cat. No. 302 217 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 400 gallery  
IHC-P: not tested yet
Clone 37B5
Subtype IgG1
Immunogen Synthetic peptide corresponding to AA 443 to 449 from rat α-Tubulin 1A (UniProt Id: P68370-1)
Epitop Epitop: AA 443 to 449 from rat α-Tubulin 1A (UniProt Id: P68370-1)
Reactivity Reacts with: mammals, chicken.
Other species not tested yet.
Specificity Specific for α-tubulin.
Data sheet 302_217.pdf

References for alpha-Tubulin - 302 217

Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 217 WB, ICC; tested species: rat
Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors.
Luchkina NV, Huupponen J, Clarke VR, Coleman SK, Keinänen K, Taira T, Lauri SE
Proceedings of the National Academy of Sciences of the United States of America (2014) 11111: 4321-6. 302 217 WB; tested species: mouse,rat
Ongoing intrinsic synchronous activity is required for the functional maturation of CA3-CA1 glutamatergic synapses.
Huupponen J, Molchanova SM, Lauri SE, Taira T
Cerebral cortex (New York, N.Y. : 1991) (2013) 2311: 2754-64. 302 217 WB; tested species: rat
Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 217 WB, ICC; tested species: rat
Cat. No.: 302 217
Amount: 100 µg
Price: $415.00
Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 217 WB, ICC; tested species: rat
Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors.
Luchkina NV, Huupponen J, Clarke VR, Coleman SK, Keinänen K, Taira T, Lauri SE
Proceedings of the National Academy of Sciences of the United States of America (2014) 11111: 4321-6. 302 217 WB; tested species: mouse,rat
Ongoing intrinsic synchronous activity is required for the functional maturation of CA3-CA1 glutamatergic synapses.
Huupponen J, Molchanova SM, Lauri SE, Taira T
Cerebral cortex (New York, N.Y. : 1991) (2013) 2311: 2754-64. 302 217 WB; tested species: rat
Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 217 WB, ICC; tested species: rat
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.