Synaptic Systems - Glu-Tubulin

Cat. No.: 302 018

Amount: 50 µg

Price: $420.00

WB

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

Other products for this target

Cat. No. 302 018	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 1000 gallery
Clone	Rb1D5
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the c-terminus of human Glu-α-tubulin. (UniProt Id: Q71U36)
Epitop	AA 448 to 450 from human Glu-α-tubulin (UniProt Id: Q71U36)
Reactivity	Reacts with: human (Q71U36), rat (P68370), mouse (P68369), zebrafish, eukaryotes, other vertebrates. Other species not tested yet.
Specificity	Specific for detyrosinated α-tubulin (glu-tubulin) and polyglutamylated tubulin (also β-tubulin). No cross reaction to tyrosinated tubulin.
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 1D5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	302_018.pdf

Glu-Tubulin antibody - 302 018