Synaptic Systems

Cat. No.: 298 003

Amount: 50 µg

Price: $455.00

Hippocampal Subregions Express Distinct Dendritic Transcriptomes that Reveal Differences in Mitochondrial Function in CA2.
Farris S, Ward JM, Carstens KE, Samadi M, Wang Y, Dudek SM
Cell reports (2019) 292: 522-539.e6. 298 003 IHC; tested species: mouse

WB

ICC

IHC

Background

The enzyme cytochrome C oxidase or Complex 4 is a large heterooligomeric enzyme consisting of 13 subunits. It is localized to the inner mitochondrial membrane. It catalyzes the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane. The resulting proton gradient finaly drives ATP synthesis.

1 ) Böttinger L et al., Mol. Biol. Cell (2013)
2 ) Su X et al., Mol. Cell. Biol. (2006)
3 ) Ostermeier C et al., Curr. Opin. Struct. Biol. (1996)

Protocols

WB

IP

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 298 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 18 from mouse COX4 (UniProt Id: P19783)
Reactivity	Reacts with: rat (P10888), mouse (P13073). Other species not tested yet.
Matching control protein/peptide	298-0P
Data sheet	298_003.pdf

COX4 antibody - 298 003

References for COX4 - 298 003