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ChAT antibody - 297 108

ChAT is an enzyme involved in the synthesis of acetylcholine
Rabbit monoclonal recombinant IgG
Cat. No.: 297 108
Amount: 50 µg
Price: $415.00
Cat. No. 297 108 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 1000 up to 1 : 2000 (see remarks) gallery  
Clone Rb110G10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 2 to 640 from rat ChAT (UniProt Id: P32738)
Reactivity Reacts with: mouse (Q03059), rat (P32738).
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 110G10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: Compared to mouse tissue, this antibody shows a poorer signal to noise ratio on rat tissue.
IHC-P: Compared to mouse tissue, this antibody shows a poorer signal to noise ratio on rat tissue.

Data sheet 297_108.pdf
Cat. No.: 297 108
Amount: 50 µg
Price: $415.00
Background
Cholinergic neurons are involved in numerous neurological functions. Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons,postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells.
Two enzymes; choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in the metabolism of ACh.