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ChAT - 297 108

An enzyme involved in the synthesis of acetylcholine
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 297 108
Amount: 50 µg
Price: 415.00 €
Cat. No. 297 108 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 1000 up to 1 : 2000 gallery  
Clone Rb110G10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 640 from rat ChAT (UniProt Id: P32738)
Reactivity Reacts with: mouse (Q03059), rat (P32738).
Other species not tested yet.
Specificity Specific for ChAT
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 110G10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 297_108.pdf
Cat. No.: 297 108
Quantity: 50 µg
Price: 415.00 €
Background
Cholinergic neurons are involved in numerous neurological functions. Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons,postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells.
Two enzymes; choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in the metabolism of ACh.