ChAT antibody - 297 015

ChAT is an enzyme involved in the synthesis of acetylcholine

Guinea pig polyclonal purified antibody

Cat. No.: 297 015

Amount: 50 µg

Price: $465.00

Cat. No. 297 015	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery iDISCO: 1 : 400 Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info.
Immunogen	Recombinant protein corresponding to AA 2 to 640 from rat ChAT (UniProt Id: P32738)
Reactivity	Reacts with: rat (P32738), mouse (Q03059). Other species not tested yet. Predicted to cross-react with human (P28329) due to high sequence homology.
Remarks	Replaces the crude Guinea pig antiserum cat. no. 297 014.
Data sheet	297_015.pdf

References for ChAT - 297 015

Ephrin-A3 is required for tonotopic map precision and auditory functions in the mouse auditory brainstem.
Hoshino N, Altarshan Y, Alzein A, Fernando AM, Nguyen HT, Majewski EF, Chen VC, Rochlin MW, Yu WM
The Journal of comparative neurology (2021) 52916: 3633-3654. 297 015 IHC; tested species: mouse

A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome.
Cursano S, Battaglia CR, Urrutia-Ruiz C, Grabrucker S, Schön M, Bockmann J, Braumüller S, Radermacher P, Roselli F, Huber-Lang M, Boeckers TM, et al.
Molecular psychiatry (2020) : . 297 015 IHC; tested species: mouse

Cat. No.: 297 015

Amount: 50 µg

Price: $465.00

IHC

IHC-P

Background

Cholinergic neurons are involved in numerous neurological functions. Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons,postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells.
Two enzymes; choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in the metabolism of ACh.

1 ) Lorenzi MV et al., DNA Cell Biol. (1992)
2 ) Luine VN et al., Exp. Neurol. (1985)

Protocols

IHC

IHC-P

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