Synaptic Systems - Myelin basic protein

Cat. No.: 295 004

Amount: 100 µl

Price: $365.00

Cat. No. 295 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 105 to 115 from rat MBP (UniProt Id: P02688)
Reactivity	Reacts with: human (P02686), rat (P02688), mouse (P04370). Other species not tested yet.
Specificity	Specific for MBP. Epitope is present in all splice variants.
Matching control protein/peptide	295-0P
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	295_004.pdf

References for Myelin basic protein - 295 004

Adult neural stem cells have latent inflammatory potential that is kept suppressed by Tcf4 to facilitate adult neurogenesis.
Shariq M, Sahasrabuddhe V, Krishna S, Radha S, Nruthyathi , Bellampalli R, Dwivedi A, Cheramangalam R, Reizis B, Hébert J, Ghosh HS, et al.
Science advances (2021) 721: . 295 004 IHC; tested species: mouse

Immunosignals of Oligodendrocyte Markers and Myelin-Associated Proteins Are Critically Affected after Experimental Stroke in Wild-Type and Alzheimer Modeling Mice of Different Ages.
Michalski D, Keck AL, Grosche J, Martens H, Härtig W
Frontiers in cellular neuroscience (2018) 12: 23. 295 004 IHC; tested species: mouse

Cat. No.: 295 004

Amount: 100 µl

Price: $365.00

IHC

Background

The myelin sheath is a multi-layered membrane composed of several proteins like PLP, claudin 11 and myelin basic protein (MBP) which is specific for the nervous system. MBP functions as an insulator and increases the velocity of axonal impulse conduction.
MBP can be subdivided into the classic group consisting of isoforms 4 to 14 and the non-classic group of MBP comprising the Golli MBPs (isoforms 1 to 3). Differential splicing events and optional posttranslational modifications give rise to a wide spectrum of isomers with potentially specialized functions.

1 ) Nawaz S et al., Glia (2013)
2 ) Harauz G et al., J. Neurochem. (2013)
3 ) Mathisen PM et al., Proc. Natl. Acad. Sci. U.S.A. (1993)
4 ) Bernard CC et al., Immunology (1981)
5 ) Dunkley PR et al., Biochem. J. (1974)

Protocols

IHC

OTHER

Preabsorbtion

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Myelin basic protein antibody - 295 004

References for Myelin basic protein - 295 004