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Home > Products > 291 002

Biotin antibody - 291 002

Biotin is a vitamin frequently used as a protein label
Rabbit polyclonal antiserum
Cat. No.: 291 002
Amount: 200 µl
Price: $360.00
Cat. No. 291 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Biotin coupled to BSA.
Reactivity Reacts with: rat, mouse.
Other species not tested yet.
Specificity This antibody recognizes biotin and desthiobiotin; BSA-specific antibodies have been removed.
Remarks

ICC: Can be used to visualize endogenous biotinylated proteins
Data sheet 291_002.pdf
Cat. No.: 291 002
Amount: 200 µl
Price: $360.00
WB
ICC
Background
Biotin is a water soluble vitamin also called vitamin B7 or vitamin H. It is essential for almost all organisms except bacteria, yeasts, molds, algae, and some plant species. Biotin is frequently conjugated to proteins and antibodies to generate suitable probes compatible with the avidin/streptavidin based detection systems.
Exemples for endogenous biotinylated proteins are biotin dependent carboxylases that mainly localize to the mitochondrial matrix. Biotin is covanlently attached to these proteins and can be employed for the visualization of mitochondria.