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Biotin - 291 002

A vitamin frequently used as a protein label
Polyclonal rabbit antiserum
Cat. No.: 291 002
Amount: 200 µl
Price: $350.00
Cat. No. 291 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Biotin coupled to BSA.
Reactivity Reacts with: rat, mouse.
Other species not tested yet.
Specificity This antibody recognizes biotin and desthiobiotin; BSA-specific antibodies have been removed.
Remarks

ICC: Can be used to visualize endogenous biotinylated proteins

Data sheet 291_002.pdf
Cat. No.: 291 002
Quantity: 200 µl
Price: $350.00
Background
Biotin is a water soluble vitamin also called vitamin B7 or vitamin H. It is essential for almost all organisms except bacteria, yeasts, molds, algae, and some plant species. Biotin is frequently conjugated to proteins and antibodies to generate suitable probes compatible with the avidin/streptavidin based detection systems.
Exemples for endogenous biotinylated proteins are biotin dependent carboxylases that mainly localize to the mitochondrial matrix. Biotin is covanlently attached to these proteins and can be employed for the visualization of mitochondria.