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Home > Products > 287 011

S100B antibody - 287 011

S100B is a marker for mature astrocytes
Mouse monoclonal purified IgG
Cat. No.: 287 011
Amount: 100 µg
Price: $415.00
Cat. No. 287 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not recommended (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 121G2F7
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity Reacts with: mouse (P50114), rat (P04631).
Other species not tested yet.
Remarks

IHC-P: Cat. no. 287 111 is recommended for this application.
Data sheet 287_011.pdf

References for S100B - 287 011

ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells.
Lu D, Zhi Y, Su H, Lin X, Lin J, Shi Y, Yi W, Hong C, Zhang T, Fu Z, Chen LY, et al.
Cell reports (2024) 433: 113818. 287 011 IHC; tested species: mouse
Cat. No.: 287 011
Amount: 100 µg
Price: $415.00
ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells.
Lu D, Zhi Y, Su H, Lin X, Lin J, Shi Y, Yi W, Hong C, Zhang T, Fu Z, Chen LY, et al.
Cell reports (2024) 433: 113818. 287 011 IHC; tested species: mouse
ICC
IHC
IHC
Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca2+-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.
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