Synaptic Systems

Cat. No.: 284 005

Amount: 50 µg

Price: $465.00

Cat. No. 284 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery EM: yes
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat DAT (UniProt Id: P23977)
Reactivity	Reacts with: rat (P23977), mouse (Q01959). Other species not tested yet.
Data sheet	284_005.pdf

References for DAT - 284 005

Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 284 005 IHC, EM; tested species: mouse

Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
Romanov RA, Zeisel A, Bakker J, Girach F, Hellysaz A, Tomer R, Alpár A, Mulder J, Clotman F, Keimpema E, Hsueh B, et al.
Nature neuroscience (2017) 202: 176-188. 284 005 IHC

Cat. No.: 284 005

Amount: 50 µg

Price: $465.00

IHC

IHC-P

Background

The dopamine transporter (DAT), encoded by the SLC6A3 gene, plays a crucial role in regulating dopaminergic neurotransmission by facilitating the reuptake of dopamine (DA) from the synaptic cleft back into presynaptic neurons (1, 2). Structurally, DAT consists of 12 transmembrane domains with interspersed extracellular and intracellular loops. The N- and C-terminal regions of the protein are intracellular, providing multiple potential phosphorylation sites that regulate its activity (1, 3).
Functionally, DAT contributes to the termination of DA signaling by reuptaking DA, thereby controlling both the duration and magnitude of dopaminergic transmission (4). It is predominantly found in dopaminergic neurons and enriched in regions such as the striatum, substantia nigra, and ventral tegmental area, which are associated with motor control and reward mechanisms (5).
Alterations in DAT expression have been linked to various neuropsychiatric disorders, including Parkinson's disease and schizophrenia, emphasizing its importance in maintaining dopaminergic homeostasis and neuronal integrity (6, 7).

1 ) Giros B et al., Nature (1996)
2 ) Amara SG et al., Drug Alcohol Depend (1998)
3 ) Freed C et al., J Comp Neurol (1995)
4 ) Amara SG et al., Annu Rev Neurosci (1993)
5 ) Bu M et al., NPJ Parkinsons Dis (2021)
6 ) Koob GF et al., Science (1997)
7 ) Fusar-Poli P et al., Schizophr Bull (2013)

Protocols

Other products for this target

DAT antibody - 284 005

References for DAT - 284 005