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Home > Products > 284 003

DAT antibody - 284 003

DAT is a neurotransmitter transporter involved in the termination of dopamine transmission
Rabbit polyclonal purified antibody
Cat. No.: 284 003
Amount: 50 µg
Price: $375.00
Cat. No. 284 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 7 to 22 from rat DAT (UniProt Id: P23977)
Reactivity Reacts with: rat (P23977), mouse (Q01959).
Other species not tested yet.
Data sheet 284_003.pdf

References for DAT - 284 003

Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 284 003 WB; tested species: mouse
Fluoxetine reverses early-life stress-induced depressive-like behaviors and region-specific alterations of monoamine transporters in female mice.
Zheng JY, Li XX, Liu X, Zhang CC, Sun YX, Ma YN, Wang HL, Su YA, Si TM, Li JT
Pharmacology, biochemistry, and behavior (2024) 237: 173722. 284 003 IHC; tested species: mouse
Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.
Liu X, Sun YX, Zhang CC, Zhang XQ, Zhang Y, Wang T, Ma YN, Wang H, Su YA, Li JT, Si TM, et al.
Neuropharmacology (2021) : 108468. 284 003 IHC; tested species: mouse
Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
Romanov RA, Zeisel A, Bakker J, Girach F, Hellysaz A, Tomer R, Alpár A, Mulder J, Clotman F, Keimpema E, Hsueh B, et al.
Nature neuroscience (2017) 202: 176-188. 284 003 IHC
Dopamine is produced in the rat spinal cord and regulates micturition reflex after spinal cord injury.
Hou S, Carson DM, Wu D, Klaw MC, Houlé JD, Tom VJ
Experimental neurology (2016) 285Pt B: 136-146. 284 003 IHC; tested species: rat
Cat. No.: 284 003
Amount: 50 µg
Price: $375.00
Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 284 003 WB; tested species: mouse
Fluoxetine reverses early-life stress-induced depressive-like behaviors and region-specific alterations of monoamine transporters in female mice.
Zheng JY, Li XX, Liu X, Zhang CC, Sun YX, Ma YN, Wang HL, Su YA, Si TM, Li JT
Pharmacology, biochemistry, and behavior (2024) 237: 173722. 284 003 IHC; tested species: mouse
Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.
Liu X, Sun YX, Zhang CC, Zhang XQ, Zhang Y, Wang T, Ma YN, Wang H, Su YA, Li JT, Si TM, et al.
Neuropharmacology (2021) : 108468. 284 003 IHC; tested species: mouse
Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
Romanov RA, Zeisel A, Bakker J, Girach F, Hellysaz A, Tomer R, Alpár A, Mulder J, Clotman F, Keimpema E, Hsueh B, et al.
Nature neuroscience (2017) 202: 176-188. 284 003 IHC
Dopamine is produced in the rat spinal cord and regulates micturition reflex after spinal cord injury.
Hou S, Carson DM, Wu D, Klaw MC, Houlé JD, Tom VJ
Experimental neurology (2016) 285Pt B: 136-146. 284 003 IHC; tested species: rat
ICC
IHC
IHC-P
Background
During neurotransmission neurotransmitters like GABA, glycine, glutamate, dopamine, etc. are released from synaptic vesicles into the synaptic cleft. The dopamine active transporter (DAT) translocates the neurotransmitter dopamine back into the cytoplasm of the neuron.
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