Synaptic Systems - alpha N-Catenin

Cat. No.: 281 103

Amount: 50 µg

Price: $375.00

WB

ICC

IHC

Background

α, β and γ-catenin are intracellular proteins that link cadherins to the actin cytosceleton. Cadherins are cell-surface proteins that are involved in cell-cell adhesion.
α-N-catenin is expressed mainly in the nervous system. It is a cytoplasmic protein that interacts with N-cadherin and functions in cell-cell adhesion. It is a regulator for the stability of synaptic contacts and is important for cerebellar and hippocampal lamination. There are two isoforms which are differentially expressed during development.
The major part of β-catenin localizes to the cell membrane and is part of E-cadherin/catenin adhesion complexes.

1 ) Taylor AM et al., J. Neurosci. (2013)
2 ) Wu H et al., Development (2012)
3 ) Nandadasa S et al., PLoS ONE (2012)
4 ) Kumamoto N et al., Nat. Neurosci. (2012)
5 ) Abe K et al., Nat. Neurosci. (2004)
6 ) Park C et al., Nat. Genet. (2002)
7 ) Benson DL et al., J. Neurosci. (1998)
8 ) Shibuya Y et al., J. Neurocytol. (1996)
9 ) Knudsen KA et al., J. Cell Biol. (1995)
10 ) Hinck L et al., J. Cell Biol. (1994)

Protocols

WB

ICC

IHC

Cat. No. 281 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 921 to 939 from mouse α N-catenin (UniProt Id: Q61301)
Reactivity	Reacts with: human (P26232), rat, mouse (Q61301). Other species not tested yet.
Specificity	Recognizes all isoforms of α-N-catenin. The antibody may cross-react to α-E-catenin due to 53 % sequence identity of the corresponding peptide sequences.
Data sheet	281_103.pdf

alpha N-Catenin antibody - 281 103