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VDAC1 antibody - 279 003

VDAC1 is a pore forming transmembrane protein
Rabbit polyclonal purified antibody
Cat. No.: 279 003
Amount: 50 µg
Price: $375.00
Cat. No. 279 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 15 to 29 from mouse VDAC1 (UniProt Id: Q60932-1)
Reactivity Reacts with: rat (Q9Z2L0), mouse (Q60932).
Other species not tested yet.
Specificity Due to sequence homologies, probably crossreacts with VDAC 2 and 3
Matching control protein/peptide 279-0P
Data sheet 279_003.pdf
Cat. No.: 279 003
Amount: 50 µg
Price: $375.00
Background
Voltage-dependent anion channels (VDACs), also known as porins, are integral membrane proteins which form a channel that allows diffusion of small hydrophilic molecules. Three closely related isoforms, VDAC 1, 2 and 3 have been described, so far.
VDACs have been originally identified as components of the outer mitochondrial membrane but they also occur in the plasma membrane of different cell types.