Aldh1L1 antibody - 278 102

Aldh1L1 is a folate metabolic enzyme

Rabbit polyclonal antiserum

Cat. No.: 278 102

Amount: 200 µl

Price: $360.00

Cat. No. 278 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: 1 : 200 up to 1 : 1000 gallery IHC-G: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Immunogen	Recombinant protein corresponding to AA 600 to 800 from mouse Aldh1l1 (UniProt Id: Q8R0Y6)
Reactivity	Reacts with: rat (P28037), mouse (Q8R0Y6). Other species not tested yet.
Specificity	Specific for Aldh1L1
Remarks	IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are recommended.
Data sheet	278_102.pdf

References for Aldh1L1 - 278 102

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 278 102 IHC; tested species: mouse

Cat. No.: 278 102

Amount: 200 µl

Price: $360.00

ICC

IHC

IHC-P

IHC-G

Background

Aldehyde dehydrogenase family 1 member L1 (Aldh1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH) is a cytosolic enzyme involved in folate metabolism. It has been shown to be involved in the regulation of cell proliferation and is downregulated in malignant human tumors and cancer cell lines.
Aldh1L1 is highly expressed in several cell-types like hepatocytes and astrocytes.

1 ) Oleinik NV et al., Genes Cancer (2011)
2 ) Yang Y et al., Glia (2011)
3 ) Rodriguez FJ et al., J. Neuropathol. Exp. Neurol. (2008)
4 ) Cahoy JD et al., J. Neurosci. (2008)
5 ) Anthony TE et al., J. Comp. Neurol. (2007)
6 ) Krupenko SA et al., Cell Growth Differ. (2002)

Protocols

ICC

IHC

IHC-P

IHC-G

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