Cat. No. 274 302
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200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
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Applications
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WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet
Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
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Immunogen
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Synthetic peptide corresponding to AA 612 to 627 from mouse GABA transporter3 (UniProt Id: P31650)
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Reactivity
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Reacts with: rat (P31647), mouse (P31650). Other species not tested yet.
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Matching control protein/peptide
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274-3P
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Remarks
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WB: GABA transporter 3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
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Data sheet
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274_302.pdf
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