Synaptic Systems - Glycine transporter2

Cat. No.: 272 008

Amount: 50 µg

Price: $415.00

WB

ICC

IHC

IHC-P

Background

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.

1 ) Kurolap A, American journal of human genetics (2016) 995: 1172-1180.
2 ) James VM, Biological chemistry (2012) 3934: 283-9.
3 ) Tsai G, Proceedings of the National Academy of Sciences of the United States of America (2004) 10122: 8485-90.
4 ) Gomeza J, Neuron (2003) 404: 785-96.
5 ) Geerlings A, The Journal of biological chemistry (2001) 27620: 17584-90.

Protocols

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ICC

IHC

IHC-P

Other products for this target

Cat. No. 272 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: yes gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 gallery
Clone	Rb117F12
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity	Reacts with: mouse (Q761V0), rat (P58295). Other species not tested yet.
Data sheet	272_008.pdf

Glycine transporter2 antibody - 272 008