Synaptic Systems

Cat. No.: 266 011

Amount: 100 µg

Price: $420.00

Cat. No. 266 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 1000 up to 1 : 5000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	350D3
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 97 from mouse NeuN (UniProt Id: Q8BIF2)
Epitop	AA 5 to 15 from mouse NeuN (UniProt Id: Q8BIF2)
Reactivity	Reacts with: rat (D4A2H6), mouse (Q8BIF2). No signal: human (A6NFN3). Other species not tested yet.
Remarks	IHC-P (FFPE): For chromogenic detection, an optimized AGR time of 5 minutes is recommended for best results. For fluorescent detection, an optimized AGR time of 5 minutes is recommended for best results. For best results apply mild antigen retrieval
Data sheet	Datasheet 266_011

References for NeuN - 266 011

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 266 011 ICC; tested species: rat

Sleep loss induces cholesterol-associated myelin dysfunction.
Simayi R, Ficiarà E, Faniyan O, Cerdán Cerdá A, Aboufares El Alaoui A, Fiorini R, Cutignano A, Piscitelli F, Maroto AS, Santos A, Del Gallo F, et al.
Proceedings of the National Academy of Sciences of the United States of America (2026) 1234: e2523438123. 266 011 IHC; tested species: rat

Oxytocin modulates respiratory heart rate variability through a hypothalamus-brainstem-heart neuronal pathway.
Buron J, Linossier A, Gestreau C, Schaller F, Tyzio R, Felix MS, Matarazzo V, Thoby-Brisson M, Muscatelli F, Menuet C
Nature neuroscience (2025) 2811: 2247-2261. 266 011 IHC; tested species: mouse

In vivo neuronal and astrocytic activation in somatosensory cortex by acupuncture stimuli.
Chang XY, Chen K, Cheng T, Lai PT, Zhang L, So KF, Yang ES
Neural regeneration research (2022) 1711: 2526-2529. 266 011 IHC; tested species: mouse

Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 266 011 IHC; tested species: mouse

Cat. No.: 266 011

Amount: 100 µg

Price: $420.00

Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 266 011 IHC; tested species: mouse

ICC

IHC

IHC-P (FFPE)

Background

NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary, and dentate nucleus neurons - NeuN is not detectable.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Morin LP et al., Brain Res. (2011)
2 ) Dent MA et al., FEBS Lett. (2010)
3 ) Cannon JR et al., Neurosci. Lett. (2009)
4 ) Kim KK et al., J. Biol. Chem. (2009)
5 ) Mullen RJ et al., Development (1992)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

NeuN antibody - 266 011

References for NeuN - 266 011