NeuN antibody - 266 008

NeuN is a neuron specific nuclear protein in vertebrates

Rabbit monoclonal recombinant IgG

Cat. No.: 266 008

Amount: 50 µg

Price: $415.00

Cat. No. 266 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 1000 up to 1 : 5000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb350D3
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 97 from mouse NeuN (UniProt Id: Q8BIF2)
Epitop	AA 5 to 15 from mouse NeuN (UniProt Id: Q8BIF2)
Reactivity	Reacts with: rat (D4A2H6), mouse (Q8BIF2). No signal: human (A6NFN3). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 350D3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-P: For best results apply short antigen retrieval (5 min, citrate pH 6,0 at 97°C).
Data sheet	266_008.pdf

References for NeuN - 266 008

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. 266 008 IHC-P; tested species: mouse

Cat. No.: 266 008

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has recently been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary and dentate nucleus neurons - NeuN is not detectable.

1 ) Morin LP et al., Brain Res. (2011)
2 ) Dent MA et al., FEBS Lett. (2010)
3 ) Cannon JR et al., Neurosci. Lett. (2009)
4 ) Kim KK et al., J. Biol. Chem. (2009)
5 ) Mullen RJ et al., Development (1992)

Protocols

ICC

IHC

IHC-P

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