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NeuN - 266 008

A neuronal specific nuclear protein in vertebrates
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 266 008
Amount: 50 µg
Price: $415.00
Cat. No. 266 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
Clone Rb350D3
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 97 from mouse NeuN (UniProt Id: Q8BIF2)
Epitop Epitop: AA 5 to 15 from mouse NeuN (UniProt Id: Q8BIF2)
Reactivity Reacts with: rat (D4A2H6), mouse (Q8BIF2).
No signal: human (A6NFN3).
Other species not tested yet.
Specificity Specific for NeuN.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 350D3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-P: For best results apply short antigen retrieval (5 min, citrate pH 6,0 at 97°C).

Data sheet 266_008.pdf
Cat. No.: 266 008
Quantity: 50 µg
Price: $415.00
Background
NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has recently been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary and dentate nucleus neurons - NeuN is not detectable.