|Cat. No. 263 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 200 up to 1 : 500 gallery
|Immunogen||Recombinant protein corresponding to AA 1 to 427 from rat Giantin (UniProt Id: G3V6A8)|
Reacts with: rat, mouse.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Specific for giantin|
WB: Due to its large size, Giantin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.