NET antibody - 260 308

The NE transporter is involved in the termination of synaptic transmission

Guinea pig monoclonal recombinant IgG

Cat. No.: 260 308

Amount: 50 µg

Price: $415.00

Cat. No. 260 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp215A9
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 200 of rat NET. (UniProt Id: Q63380)
Reactivity	Reacts with: rat (Q63380), mouse (O55192). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 215A9. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	260_308.pdf

Cat. No.: 260 308

Amount: 50 µg

Price: $415.00

IHC

Background

The norepinephrine (NE) transporter (NET), also referred to as Sodium-dependent noradrenaline transporter, is expressed on the plasma membranes of noradrenergic neurons. It is responsible for the inactivation of synaptically released NE and is a target for many antidepressants and psychostimulants.

1 ) Robertson SD et al., J. Neurosci. (2010)
2 ) Li Z et al., J. Neurosci. (2010)
3 ) Guiard BP et al., Mol. Pharmacol. (2008)
4 ) Schroeter S et al., J. Comp. Neurol. (2000)
5 ) Kuczenski R et al., J. Neurosci. (1995)

Protocols

IHC

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