Cannabinoid receptor CB1-R antibody - 258 106

CB1-R is a G-protein coupled receptor responsive to cannabinoids

Chicken polyclonal purified antibody

Cat. No.: 258 106

Amount: 50 µg

Price: $380.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 258 106	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 5000 gallery IHC-P: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 450 to 473 from rat CB1-R (UniProt Id: P20272)
Reactivity	Reacts with: mouse (P47746), rat (P20272). Other species not tested yet.
Remarks	IHC-P: Heat mediated antigen retrieval at pH 9.0-10.0 (10 mM Tris, 1 mM EDTA).
Data sheet	258_106.pdf

Cat. No.: 258 106

Amount: 50 µg

Price: $380.00

IHC

IHC-P

Background

The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.

1 ) Osborne KD et al., ASN Neuro (2009)
2 ) Niehaus JL et al., Mol. Pharmacol. (2007)
3 ) Kawamura Y et al., J. Neurosci. (2006)
4 ) Shire D et al., J. Biol. Chem. (1995)
5 ) Matsuda LA et al., Nature (1990)
6 ) Howlett AC et al., Mol. Pharmacol. (1985)

Protocols

IHC

IHC-P

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