Cannabinoid receptor CB1-R antibody - 258 009 K.O.

CB1-R is a G-protein coupled receptor responsive to cannabinoids

Chicken monoclonal recombinant IgY

Cat. No.: 258 009

Amount: 50 µg

Price: $415.00

Cat. No. 258 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet Dot blot: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunodetection of target molecules spotted onto a membrane. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch289C1
Subtype	IgY (λ light chain)
Immunogen	Synthetic peptide corresponding to AA 450 to 473 from rat CB1-R (UniProt Id: P20272)
Reactivity	Reacts with: rat (P20272), mouse (P47746), human (P21554). Other species not tested yet.
Specificity	K.O. validated
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 289C1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	258_009.pdf

Cat. No.: 258 009

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.

1 ) Osborne KD et al., ASN Neuro (2009)
2 ) Niehaus JL et al., Mol. Pharmacol. (2007)
3 ) Kawamura Y et al., J. Neurosci. (2006)
4 ) Shire D et al., J. Biol. Chem. (1995)
5 ) Matsuda LA et al., Nature (1990)
6 ) Howlett AC et al., Mol. Pharmacol. (1985)

Protocols

ICC

IHC

IHC-P

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