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Home > Products > 258 008

Cannabinoid receptor CB1-R antibody - 258 008 K.O.

CB1-R is a G-protein coupled receptor responsive to cannabinoids
Rabbit monoclonal recombinant IgG
Cat. No.: 258 008
Amount: 50 µg
Price: $420.00
Cat. No. 258 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone Rb289C1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 450 to 473 from rat CB1-R (UniProt Id: P20272)
Reactivity Reacts with: rat (P20272), mouse (P47746), human (P21554).
Other species not tested yet.
Specificity K.O. validated PubMed: 36933217
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 289C1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet 258_008.pdf

References for Cannabinoid receptor CB1-R - 258 008

Somatic and terminal CB1 receptors are differentially coupled to voltage-gated sodium channels in neocortical neurons.
Steiger LJ, Tsintsadze T, Mattheisen GB, Smith SM
Cell reports (2023) 423: 112247. 258 008 ICC; KO verified; tested species: mouse
Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice.
Brito DVC, Kupke J, Sokolov R, Cambridge S, Both M, Bengtson CP, Rozov A, Oliveira AMM
Molecular psychiatry (2024) : . 258 008 IHC; tested species: mouse
Increased hippocampal cannabinoid 1 receptor expression is associated with protection from severe seizures in pregnant mice with reduced uterine perfusion pressure.
Jones-Muhammad M, Pryor T, Shao Q, Freeman KB, Warrington JP
Journal of neuroscience research (2023) : . 258 008 IHC; tested species: mouse
Cat. No.: 258 008
Amount: 50 µg
Price: $420.00
Somatic and terminal CB1 receptors are differentially coupled to voltage-gated sodium channels in neocortical neurons.
Steiger LJ, Tsintsadze T, Mattheisen GB, Smith SM
Cell reports (2023) 423: 112247. 258 008 ICC; KO verified; tested species: mouse
Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice.
Brito DVC, Kupke J, Sokolov R, Cambridge S, Both M, Bengtson CP, Rozov A, Oliveira AMM
Molecular psychiatry (2024) : . 258 008 IHC; tested species: mouse
Increased hippocampal cannabinoid 1 receptor expression is associated with protection from severe seizures in pregnant mice with reduced uterine perfusion pressure.
Jones-Muhammad M, Pryor T, Shao Q, Freeman KB, Warrington JP
Journal of neuroscience research (2023) : . 258 008 IHC; tested species: mouse
ICC
IHC
IHC
IHC-P
IHC-P
Background
The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.
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