Synaptic Systems - TARP-gamma3

Cat. No.: 254 003

Amount: 50 µg

Price: $380.00

WB

ICC

Background

Ligand gated cation channels of the AMPA subtype are composed of different GluA subunits and interact with a family of proteins termed transmembrane AMPA-receptor regulatory proteins (TARPs) also known as voltage gated calcium channel gamma subunits or CNAGs (1). These proteins regulate the surface expression and biophysical properties like the gating time of AMPA glutamate receptors (1). Six transmembrane AMPA receptor regulatory protein (TARP) isoforms, classified as Type I (γ-2, -3, -4, and -8) and Type II (γ-5 and -7), are discretely expressed in specific neuronal and glial populations and differentially regulate synaptic transmission throughout the brain (2). One key feature of TARP-gamma2 and TARP-gamma8 is the differential regulation of synaptic strength in the hippocampus that is determined by the number and density of AMPA glutamate receptors (3).
Especially, TARP-gamma8 shows high expression levels in the hippocampus and is only sparsely expressed in other brain areas (4).
The TARP-gamma3 gene locus shows a linkage to childhood absence epilepsy (CAE) (5).

1 ) Shi Y et al., Proc. Natl. Acad. Sci. U.S.A. (2010)
2 ) Kato AS et al., Neuron (2010)
3 ) Yamasaki M et al., J Neurosci (2016)
4 ) Fukaya M et al., Eur J Neurosci (2006)
5 ) Everett KV et al., Eur J Hum Genet (2007)

Protocols

WB

ICC

Other products for this target

Cat. No. 254 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 294 to 307 from mouse TARP-γ3 (UniProt Id: Q9JJV5)
Reactivity	Reacts with: rat (Q8VHX0), mouse (Q9JJV5). Other species not tested yet.
Remarks	ICC: This antibody produces some unspecific somatic background staining on cell bodies in addition to specific labeling of post-synaptic terminals.
Data sheet	254_003.pdf

TARP-gamma3 antibody - 254 003