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beta-Actin - 251 008

A major constituent of the cytoskeleton
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 251 008
Amount: 50 µg
Price: $415.00
Cat. No. 251 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone Rb130B4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Epitop Epitop: AA 2 to 14 from mouse β-Actin (UniProt Id: P60710)
Reactivity Reacts with: rat (P60711), mouse (P60710), human (P60709), zebrafish.
Other species not tested yet.
Specificity May cross-react to α- and γ-actin due to sequence homology.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 130B4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
ICC: To avoid precipitates, the antibody should be centrifuged before staining.

Data sheet 251_008.pdf
Cat. No.: 251 008
Quantity: 50 µg
Price: $415.00
Background
The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.