beta-Actin antibody - 251 008

beta-Actin is a major constituent of the cytoskeleton

Rabbit monoclonal recombinant IgG

Cat. No.: 251 008

Amount: 50 µg

Price: $415.00

Cat. No. 251 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 2000 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb130B4
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity	Reacts with: rat (P60711), mouse (P60710), human (P60709), zebrafish. Other species not tested yet.
Specificity	May cross-react to α- and γ-actin due to sequence homology.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 130B4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. ICC: To avoid precipitates, the antibody should be centrifuged before staining.
Data sheet	251_008.pdf

Cat. No.: 251 008

Amount: 50 µg

Price: $415.00

ICC

Background

The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.

1 ) Bassell GJ et al., J. Neurosci. (1998)
2 ) Ng SY et al., Nucleic Acids Res. (1989)
3 ) Nudel U et al., Nucleic Acids Res. (1983)
4 ) Czosnek H et al., EMBO J. (1983)

Protocols

Standard protocol

ICC

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