Synaptic Systems

Cat. No.: 250 403

Amount: 50 µg

Price: $380.00

WB

Background

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. After the release of glutamate from synaptic vesicles into the synaptic cleft during neurotransmission, excitatory amino acid transporters (EAATs) remove extracellular glutamate to avoid excitotoxic levels (1).
Five EAATs with differential expression patterns have been described so far: EAAT1, also referred to as GLAST and SLC1A3, has neuroprotective potential following ischemia and occurs in reactive astrocytes and activated microglia. EAAT2 (GLT-1, SLC1A2) is the most abundant isoform and is primarily expressed in astrocytes. Both variants show high levels in brain and retina. EAAT3 / SLC1A1, EAAT4 / SLC1A6 and EAAT5 / SLC1A7 are expressed in neurons (2). EAAT4 shows weak expression in the forebrain and high levels in the cerebellum, where it mainly locates to Purkinje cells (3). EAAT5 primarily occurs in the retina, where it locates very close to glutamate release sites. In K.O. mice flicker resolution is considerably compromised (4). Recent findings suggest that EAAT5 is an abundant isoform, expressed also in non-neuronal peripheral tissues (5).

1 ) Martins IJ et al., Biochim Biophys Acta (1989)
2 ) Butchbach ME et al., J Biol Chem (2004)
3 ) Malhotra S et al., J Neurosci (2021)
4 ) Gehlen J et al., eNeuro (2021)
5 ) Lee A et al., Eur J Histochem (2013)

Protocols

WB

Other products for this target

Cat. No. 250 403	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not recommended (see remarks) IHC: not recommended (see remarks) IHC-P: not tested yet (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse EAAT4 (UniProt Id: O35544)
Reactivity	Reacts with: mouse (O35544), rat (O35921). Other species not tested yet.
Remarks	ICC: Cat. no. 250 413 is the first choice for immunocytochemistry experiments. IHC: Cat. no. 250 413 is the first choice for immunohistochemistry experiments. IHC-P: Cat. no. 250 413 is the first choice for immunohistochemistry experiments on FFPE tissue.
Data sheet	250_403.pdf

EAAT4 antibody - 250 403