Tailor-made Antibodies
and Tools for Life Science

EAAT2 antibody extracellular domain - 250 203 K.O.

EAATs are transmembrane proteins involved in the removal of extracellular glutamate
Rabbit polyclonal purified antibody
Cat. No.: 250 203
Amount: 50 µg
Price: $385.00
Cat. No. 250 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: yes (see remarks) gallery  
IHC: 1 : 1000 up to 1 : 2000 (see remarks) gallery  
IHC-P: 1 : 500 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to AA 146 to 161 from mouse EAAT2 (UniProt Id: P43006)
Reactivity Reacts with: rat (P31596), mouse (P43006).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 250-2P
Remarks

ICC: This antibody yields excellent results on mouse cells, but is not recommended for rat samples, as it produces non-specific nuclear staining in those specimens.
IHC: This antibody yields excellent results on mouse tissue but is not recommended for rat samples, as it produces non-specific nuclear staining in those specimens.

Fixation with 4% PFA and 0,1% glutaraldehyde according to Danbolt et al. 1998 is recommended.
IHC-P: This antibody yields excellent results on mouse tissue but is not recommended for rat samples, as it produces non-specific nuclear staining in those specimens.

Data sheet 250_203.pdf

References for EAAT2 - 250 203

Depressed glutamate transporter 1 expression in a mouse model of Dravet syndrome.
Hameed MQ, Hui B, Lin R, MacMullin PC, Pascual-Leone A, Vermudez SAD, Rotenberg A
Annals of clinical and translational neurology (2023) 109: 1695-1699. 250 203 WB; tested species: mouse
Rapid recycling of glutamate transporters on the astroglial surface.
Michaluk P, Heller JP, Rusakov DA
eLife (2021) 10: . 250 203 WB; tested species: mouse
Astrocyte dysfunction increases cortical dendritic excitability and promotes cranial pain in familial migraine.
Romanos J, Benke D, Pietrobon D, Zeilhofer HU, Santello M
Science advances (2020) 623: eaaz1584. 250 203 WB; tested species: mouse
Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation.
Romanos J, Benke D, Saab AS, Zeilhofer HU, Santello M
Communications biology (2019) 2: 127. 250 203 WB; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 250 203 WB, IHC; tested species: mouse
Strategies for immunohistochemical protein localization using antibodies: What did we learn from neurotransmitter transporters in glial cells and neurons.
Danbolt NC, Zhou Y, Furness DN, Holmseth S
Glia (2016) 6412: 2045-2064. 250 203 WB
Cat. No.: 250 203
Amount: 50 µg
Price: $385.00