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Home > Products > 248 003

Mover antibody - 248 003 K.O.

Mover is a presynaptic protein
Rabbit polyclonal purified antibody
Cat. No.: 248 003
Amount: 50 µg
Price: $375.00
Cat. No. 248 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 100 gallery  
IHC-P: not tested yet
DNA-PAINT: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Immunogen Recombinant protein corresponding to AA 1 to 266 from rat Mover (UniProt Id: A8WCF8)
Reactivity Reacts with: rat (A8WCF8), mouse (Q9DBS2).
Other species not tested yet.
Specificity Specific for mover. Shows some cross-reactivity to Ribeye K.O. validated PubMed: 31787876
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet 248_003.pdf

References for Mover - 248 003

The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 248 003 WB, ICC; KO verified; tested species: mouse
Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
Körber C, Horstmann H, Venkataramani V, Herrmannsdörfer F, Kremer T, Kaiser M, Schwenger DB, Ahmed S, Dean C, Dresbach T, Kuner T, et al.
Neuron (2015) 873: 521-33. 248 003 WB, IHC; KD verified
The synaptic vesicle protein Mover/TPRG1L is associated with lipid droplets in astrocytes.
Krohn J, Domart F, Do TT, Dresbach T
Glia (2023) : . 248 003 ICC, IHC; tested species: mouse
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 248 003 WB, ICC; KO verified; tested species: mouse
The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein.
Riemann D, Wallrafen R, Dresbach T
Scientific reports (2017) 71: 15791. 248 003 ICC; tested species: rat
The synaptic vesicle protein Mover/TPRG1L is associated with lipid droplets in astrocytes.
Krohn J, Domart F, Do TT, Dresbach T
Glia (2023) : . 248 003 ICC, IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 248 003 IHC; tested species: rat
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence.
Wallrafen R, Dresbach T, Viotti JS
Journal of visualized experiments : JoVE (2019) 143: . 248 003 IHC; tested species: mouse
The Presynaptic Protein Mover Is Differentially Expressed Across Brain Areas and Synapse Types.
Wallrafen R, Dresbach T
Frontiers in neuroanatomy (2018) 12: 58. 248 003 IHC; tested species: mouse
Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
Körber C, Horstmann H, Venkataramani V, Herrmannsdörfer F, Kremer T, Kaiser M, Schwenger DB, Ahmed S, Dean C, Dresbach T, Kuner T, et al.
Neuron (2015) 873: 521-33. 248 003 WB, IHC; KD verified
Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse.
Mendoza Schulz A, Jing Z, Sánchez Caro JM, Wetzel F, Dresbach T, Strenzke N, Wichmann C, Moser T
The EMBO journal (2014) 335: 512-27. 248 003 IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 248 003 DNA-PAINT; tested species: rat
Cat. No.: 248 003
Amount: 50 µg
Price: $375.00
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 248 003 WB, ICC; KO verified; tested species: mouse
Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
Körber C, Horstmann H, Venkataramani V, Herrmannsdörfer F, Kremer T, Kaiser M, Schwenger DB, Ahmed S, Dean C, Dresbach T, Kuner T, et al.
Neuron (2015) 873: 521-33. 248 003 WB, IHC; KD verified
The synaptic vesicle protein Mover/TPRG1L is associated with lipid droplets in astrocytes.
Krohn J, Domart F, Do TT, Dresbach T
Glia (2023) : . 248 003 ICC, IHC; tested species: mouse
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 248 003 WB, ICC; KO verified; tested species: mouse
The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein.
Riemann D, Wallrafen R, Dresbach T
Scientific reports (2017) 71: 15791. 248 003 ICC; tested species: rat
The synaptic vesicle protein Mover/TPRG1L is associated with lipid droplets in astrocytes.
Krohn J, Domart F, Do TT, Dresbach T
Glia (2023) : . 248 003 ICC, IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 248 003 IHC; tested species: rat
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence.
Wallrafen R, Dresbach T, Viotti JS
Journal of visualized experiments : JoVE (2019) 143: . 248 003 IHC; tested species: mouse
The Presynaptic Protein Mover Is Differentially Expressed Across Brain Areas and Synapse Types.
Wallrafen R, Dresbach T
Frontiers in neuroanatomy (2018) 12: 58. 248 003 IHC; tested species: mouse
Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover.
Körber C, Horstmann H, Venkataramani V, Herrmannsdörfer F, Kremer T, Kaiser M, Schwenger DB, Ahmed S, Dean C, Dresbach T, Kuner T, et al.
Neuron (2015) 873: 521-33. 248 003 WB, IHC; KD verified
Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse.
Mendoza Schulz A, Jing Z, Sánchez Caro JM, Wetzel F, Dresbach T, Strenzke N, Wichmann C, Moser T
The EMBO journal (2014) 335: 512-27. 248 003 IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 248 003 DNA-PAINT; tested species: rat
WB
ICC
IHC
Background

Mover, also referred to as TPRG1L, is a presynaptic protein that is enriched in the synaptic vesicle fraction. In vivo studies reveal co-localization of mover with classical pre-synaptic markers like synaptotagmin 1, synaptophysin and bassoon.
Depending on the brain region examined mover differentially localizes to inhibitory or exitatory subsets of synapses.